IMUBIND® vWF ELISA REF: 828
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The IMUBIND® vWF ELISA is an enzyme-linked immunoassay for the measurement of vWF antigen in human plasma.Von Willebrand Factor (vWF) is a large, multimeric protein (molecular weight of 1,000-20,000 kD) composed of repeating 270 kD subunits containing 2050 amino acid residues. It is synthesized by endothelial cells and megakaryocytes, and is present in multimeric form in the basement membrane of the subendothelium, in plasma and platelets. vWF promotes platelet adhesion to damaged endothelium and is a key component in the formation of the platelet plug and stable clot formation. Together with fibronectin and collagen, vWF functions in maintaining vessel wall integrity. vWF also functions as a carrier protein for Factor VIII, the coagulation protein absent in haemophilia A. Von Willebrand disease is a bleeding disorder characterized by decreased levels of circulating vWF protein (Type 1), a complete absence of vWF protein (Type 3), and low vWF activity (Types 2A, 2B, 2M, 2N).
The IMUBIND® vWF ELISA is an enzyme-linked immunoassay for the measurement of vWF antigen in human plasma.Von Willebrand Factor (vWF) is a large, multimeric protein (molecular weight of 1,000-20,000 kD) composed of repeating 270 kD subunits containing 2050 amino acid residues. It is synthesized by endothelial cells and megakaryocytes, and is present in multimeric form in the basement membrane of the subendothelium, in plasma and platelets. vWF promotes platelet adhesion to damaged endothelium and is a key component in the formation of the platelet plug and stable clot formation. Together with fibronectin and collagen, vWF functions in maintaining vessel wall integrity. vWF also functions as a carrier protein for Factor VIII, the coagulation protein absent in haemophilia A. Von Willebrand disease is a bleeding disorder characterized by decreased levels of circulating vWF protein (Type 1), a complete absence of vWF protein (Type 3), and low vWF activity (Types 2A, 2B, 2M, 2N).
| REAGENTS | |
|---|---|
| 96 | Anti-Human vWF IgG Coated Microwells with an acetate cover sheet |
| 6 | vials of vWF standards, 0 – 10 mU/mL (lyophilized) |
| 1 | vial of Detection Antibody, HRP-conjugated anti-human vWF, 135 μL |
| 1 | vial of Detection Antibody Diluent, 20 mL (lyophilized) |
| 1 | vial of Substrate, TMB, 11 mL |
| 1 | packet of Wash Buffer, PBS with 0.05% Tween 20, pH 7.4, 1 L (lyophilized) |
| SPECIFICATIONS | |
|---|---|
| SAMPLES | Citrate or EDTA collected plasma |
| SAMPLE PREPARATION | 1:100 dilution |
| SAMPLE VOLUME | 100 μL diluted plasma |
| TOTAL ASSAY TIME | 3 hours |
| STANDARD RANGE | 0 – 10 mU/mL (0.01 IU/mL) |
| LOWER LIMIT OF DETECTION | 0.1 Mu/Ml (1 IU/mL) |
| PRECISION | N.D. |
| NUMBER OF TESTS | 96 |